3021D
Category:
Product Specifications
500mL, 50mL*10
All products are manufactured using specialized equipment and strictly in accordance with GMP standards to ensure high quality and reduce batch-to-batch variations.
GMP standards
Keywords:
3021D
Product Inquiry
3021D
Scope of Application
| Basic Cell Culture Supports the growth of various adherent and suspension cells (such as HEK293, Vero, CHO, MSC, etc.) Provides essential nutrients such as growth factors, hormones, and proteins |
Primary Cell Culture Used for the isolation and culture of primary cells (such as hepatocytes, neurons, fibroblasts) |
| Stem Cell Culture Proliferation and maintenance of embryonic stem cells (ESCs) and mesenchymal stem cells (MSCs) |
In Vitro Experimental Models 3D cell culture, organoid culture, tumor cell models, etc. |
| Drug Toxicity Testing Evaluate the effects of compounds on cell viability and apoptosis (such as MTT assay) |
Gene Editing Research Recovery culture of cells after CRISPR/Cas9 transfection |
| Cryopreservation As a component of cell cryopreservation solutions (such as DMSO + serum), reducing ice crystal damage |
Wound Healing Research Growth factor-rich serum (such as fetal bovine serum) is used in skin regeneration experiments |
| Catalog Number | 3021D |
| Product Specifications | 500mL, 50mL*10 |
| Blood Source | South America |
Application Cases
RAW264.7 cells
Passage method: Cells were seeded at 4x10³ cells per passage in T25 cell culture flasks and passaged every 3 days for 6 consecutive passages.
Culture system: DMEM + 10% FBS T25 cell culture flask
Culture conditions: 37℃ 5%CO2
Cell State Diagram
|
Umedium- South America (P3, 4x, 72h) |
G brand- South America (P3, 4x, 72h) |
Umedium- South America (P6, 4x, 72h) |
G brand- South America (P6, 4x, 72h) |
Passage method: Cells were seeded at a concentration of 2x10⁵ cells/mL per passage, and counted daily to plot a growth curve.
Culture system: DMEM + 10% FBS 12-well plate
Culture conditions: 37℃ 5%CO2
Application Cases
MSC cells
Passage method: Cells were seeded at 3x10⁵ cells per passage in T25 cell culture flasks and passaged every 3 days for 6 consecutive passages.
Culture system: α-MEM + 10% FBS T25 cell culture flask
Culture conditions: 37℃ 5%CO2
Cell State Diagram
|
Umedium- South America (P3, 4x, 72h) |
G brand- South America (P3, 4x, 72h) |
Umedium- South America (P6, 4x, 72h) |
G brand- South America (P6, 4x, 72h) |
Passage method: Cells were seeded at a concentration of 1x10⁵ cells/mL per passage, and counted daily to plot a growth curve.
Culture system: α-MEM + 10% FBS 6-well plate
Culture conditions: 37℃ 5%CO2
Application Cases
293T cells
Passage method: Cells were seeded at 5x10⁵ cells per passage in T25 cell culture flasks and passaged every 3 days for 6 consecutive passages.
Culture system: DMEM + 10% FBS T25 cell culture flask
Culture conditions: 37℃ 5%CO2
Cell State Diagram
|
Umedium- South America (P3, 4x, 72h) |
G brand- South America (P3, 4x, 72h) |
Umedium- South America (P6, 4x, 72h) |
G brand- South America (P6, 4x, 72h) |
Passage method: Cells were seeded at a concentration of 2x10⁵ cells/mL per passage, and counted daily to plot a growth curve.
Culture system: DMEM + 10% FBS 12-well plate
Culture conditions: 37℃ 5%CO2
Application Cases
THP-1 cells
Passage method: Cells were seeded at 5x10⁵ cells per passage in T25 cell culture flasks and passaged every 3 days for 6 consecutive passages.
Culture system: RPMI-1640 + 10% FBS + 0.05mM β-mercaptoethanol T25 cell culture flask
Culture conditions: 37℃ 5%CO2
Cell State Diagram
|
Umedium- South America (P3, 4x, 72h) |
G brand- South America (P3, 4x, 72h) |
Umedium- South America (P6, 4x, 72h) |
G brand- South America (P6, 4x, 72h) |
Passage method: Cells were seeded at a concentration of 2x10⁵ cells/mL per passage, and counted daily to plot a growth curve.
Culture system: RPMI-1640 + 10% FBS + 0.05mM β-mercaptoethanol 12-well plate
Culture conditions: 37℃ 5%CO2
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