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3021A

Product Specifications

500ml

Comprehensive physicochemical and microbiological testing, strictly in accordance with pharmacopoeia standards, stable quality, low endotoxin content, low hemoglobin content and other physicochemical properties

Stable quality

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3021A


product Product Inquiry

3021A


Product Features

Comprehensive physicochemical and microbiological testing, strictly in accordance with pharmacopoeia standards, stable quality, low endotoxin content, low hemoglobin content and other physicochemical properties.

Product Advantages

Validated by multiple cells, with clear cell background, good cell state, and high cell proliferation rate.

Scope of Application

Suitable for stem cell, immune cell, and primary cell culture. For example: mESC, MSC, iPSC, etc.

 

Product Information

Product Catalog Number 3021A Shipping Method Dry ice, cold chain
Product Specifications 500ml, 50ml*10 Endotoxin Content ≤1EU/ml
Blood origin  China Hemoglobin Content ≤200mg/L
Storage Conditions (-10~-30)℃ Protein Content 30-45g/L
Applicable Types Stem Cells 
Immune Cells 
Primary Cells
Biosafety Monitoring No bacteria, fungi 
No mycoplasma 
No viral contamination

 

Application Cases

Mouse embryonic stem cells C57BL/6J, 2i culture system

Culture Method

Using primary isolated mouse embryonic stem cells P3 generation as starting cells, the inoculation amount for each passage of the two culture media is the same and is 2.0-4.0 x 105 /2ml;

Control serum: G brand, Australia

Cell Count

3021A(x10⁶) Control(x10⁶) Generation
2.15 2.17 2.16 2.13 P1
2.12 2.03 2.13 2.14 P2
4.22 4.23 4.08 4.15 P3
4.57 4.33 4.18 4.12 P4
4.57 4.54 4.42 4.60 P5
7.78 7.62 5.45 5.55 P6

Note: Cell death rate is not recorded, the inoculation amount is consistent, the yield, cell 3D state and fluorescence expression represent the cell state.

 

After digesting P2 generation monoclonal cells, they were inoculated in 2i medium to obtain P3 generation embryonic stem cells, which are recorded as the P0 generation of this passage. The edges are clear, 3D growth, round colony spots, and strong fluorescence signal can be seen under fluorescence.

 

P6: mESC cells cultured with 3021A, high colony formation rate, round colony shape, obvious 3D raised state structure, no decrease in yield and normal growth.

3021A                                   G brand

P6-Fluorescence 4x

3021A                                     G brand

P6-Fluorescence 10x

3021A                                  G brand

P6-Bright Field 4x

3021A                                  G brand

P6-Bright Field 10x

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